Chapter 4 Task Completion

Zhou Wen According to the experimental procedure, pipette the supernatant.

“Blowing” is an academic term, which is to extract the supernatant with a test tube gun and then blow it out. The purpose is to fully stir the clear liquid in the test tube evenly.

Pipette back and forth several times, then extract the two DNA samples into the centrifuge and start centrifugation.

The centrifugation is complete after fifteen minutes.

Take the supernatant of group A to the side of the electrophoresis machine and add it from the gel well.

Cover the electrophoresis tank cover, select the electrophoresis voltage and direction, turn on the power switch, and start electrophoresis.

Followed by group B, the supernatant was also extracted, added from the gel well of another electrophoresis machine, and the same was run for electrophoresis.

Then there is a long wait.

The computer will compare the extracted DNA with the mouse genes in the “NCBI Gene Bank” in the United States one by one, and it will take at least 6 hours.

Because there are 2.5 billion DNA base pairs on the 20 pairs of chromosomes in white mice, it takes a long time.

During this time, you can read novels, play dramas, and eat melon seeds.

But Zhou Wen has no time.

Go and grab a little white mouse and come over, first observe it with the eyes of true vision.

What made him didn’t expect was that what appeared in front of him this time was an introduction, [rat name: little white mouse, a variant of mole. Gender: female. Rat age: 11 months.

“Hey, why did you see this?” Zhou Wen turned his head and looked at Lin Jiayi, who was facing away from him. The scene he saw before appeared again.

Name: Lin Jiayi. Gender: Female. Age: 24. Occupation: Biology student. body condition:? ? ? Mental state:? ? ?

He thought of the way to observe DNA, his eyes narrowed slightly, and soon the picture in front of him began to become clearer and clearer…

” Fuck… Fuck…”

Lin Jiayis upper body white lab coat slowly turned into fibrous tissue in his eyes, spreading, increasing… Continue to move forward, it is white chemical fiber fabric .

The pores of the chemical fiber fabric became thicker and thicker in front of my eyes, and finally passed through this layer of chemical fiber fabric and turned into a black cotton fabric.

Next is the epidermal keratinization layer, dermis, arteries, veins, hair follicles…

At this moment, Lin Jiayi suddenly turned around and asked: “Zhou Wen, you What are you looking at?”

Zhou Wen was interrupted, the picture in front of him disappeared, came back to his senses, rubbed his nose and said, “Uh… you didn’t look at me, how do you know I was looking at you? Huh?”

Lin Jiayi pointed to the stainless steel frame of the aseptic operating table next to it, and Zhou Wen’s reflection can be clearly seen on it.

Lin Jiayi laughed at hehe and asked: “Hey Zhou Wen, you are so kind to help Shi Lei do experiments, isn’t it just to stay with me?”

Zhou Wen: “… _hy do you have such unrealistic ideas?”

Since Qu Fangfang left, Lin Jiayi probably felt that no one in the laboratory could threaten her appearance, so her words became more and more abandon all restraint.

Of course, Lin Jiayi looks good, with a cheeky face, a figure with a figure, and the smaller white lab coat is tightly supported.

Its a group of reality, looking for a handsome boyfriend.

And I’m so good at plot against, a bit disgusting.

Lin Jiayi smiled and said: “Hey~ It doesn’t matter if you don’t admit it… Forget it, I have a boyfriend, and you are too late even if you want to chase me.”

Zhou Wen turned around and choked off the neck of the little white mouse, and said with a smile, “Congratulations on taking off the order. Tell me, what’s the situation with the other party?”

“He Its also from our school, one year younger than me, a native of Jiang Prefecture, who studied CS……”

As Lin Jiayi was talking, Zhou Wens attention was mostly on the mouse in his hand. Body.

He found that when he took out the bone marrow from the white rat’s thigh, he could already see the DNA chain.

This is much easier, he can see the possible problems in the DNA extraction process more intuitively.

Soon he discovered that in the process of flushing with low osmotic fluid, a lot of DNA fragments were lost.

I wonder if it is caused by the low osmotic fluid concentration problem?

But fortunately, it does not affect the extraction of genes, and the concentration issues will be studied later.

Next is the counterweight centrifugation.

Zhou Wen centrifugal test of plant by plant.

Soon he found the reason for the failure of group B extraction, which was a problem in the DNA extraction process.

This is a common problem for novices. Others such as temperature control, time control, techniques, etc., will affect the number of DNA strands extracted and the success rate.

Under normal circumstances, there is no way. There is a difference between left-hand extraction and right-hand extraction. Centrifugation is one second or less, it may fail, and the temperature is higher and lower. Will have a big impact.

Because DNAnaked eye is invisible and intangible, novices can only find out possible problems through repeated experiments. That is to say, rely on “experience”.

But now Zhou Wen has true vision, he can see DNA directly, the result is completely different.

He stepped through the process of accumulating experience and became a top expert directly……

……

Time passed quickly, and it was already night in a blink of an eye It’s half past five.

Lin Jiayi stood up and stretched her waist, beat her neck and walked to Zhou Wen and said: “Hey Zhou Wen, do you want to go to dinner?”

“Gum” Zhou Wen, without raising his head, said: “You go first, my side will be ready soon.” Lin Jiayi didn’t notice, there was a layer of little white mouse in the waste bucket next to it. The corpse, nodded and said: “Okay, then I’m leaving.”

“Hmm…”

The electrophoresis analysis was not completed until 7:30 in the evening.

After comparison, it was found that group A was successfully extracted, while group B was not unexpected. The data was seriously unreasonable, indicating that the extraction really failed.

Zhou Wen looked at the data chart and smiled uncontrollably at the corner of his mouth.

The task required 6 gene fragments. In the afternoon, he extracted 8 strains. Among them, 3 strains were obtained after electrophoresis analysis, 1 strain was obtained after electron microscope observation, and 4 strains were obtained through naked identified by eye.

At this moment, a soft voice rang in my ear, just like a voice actor.

[The task conditions have been fulfilled, do you want to submit the task immediately?

After Zhou Wen froze for a while, she was immediately overjoyed and immediately turned on the pharmacist system.

On the blue attribute board, a prompt box pops up, and it says [Do you want to submit the task immediately? Yes/No]

Zhou Wen selectsYes

Ding! Task completed. Now start to issue rewards… Congratulations on getting 100 subject experience and 200 points. ]

After giving out the experience, a suggestion box pops up again, [Do you want to open the box now? Yes/No]

Zhou Wen was initially a little excited, but as the opening prompt popped up, she became excited.

“God bless you, open a million…no, 10,000 will do. It really doesn’t work for 1,000 yuan.”

He is really short of money now, and all his belongings are left. 100 yuan.

Today is only March 19th, and the 100 yuan will last until the beginning of next month.

In fact, when he came to school in the next year, he had two thousand yuan on his body.

As a result, the phone broke accidentally. I spent 1,000 to buy a three-handed 7P from Junior Brother, and then recharged another 600 meal cards. I bought some things in bits and pieces. The next 100 yuan.

Zhou Wen prayed silently in her heart, and then chose [Yes]

Just after choosing, a black lacquered wooden texture treasure box popped out in front of him, with complicated patterns engraved on it , There is an old key floating beside it.

“It’s kind of like that~” Zhou Wen rubbed his hands in excitement, and then muttered: “Luck Emperor possesses the body, the non-Chief retreats…”

[Open]

As the words fall, the key floating next to the treasure chest is automatically inserted into the keyhole of the treasure chest.

After spinning a circle, the treasure chest slowly opened, and an object shining with white radiance emerged.

Before Zhou Wen can see clearly what it is, a prompt box pops up.

He took a closer look, [Congratulations, you got the bio-type N99 mask10…]

(End of this chapter)


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