Supreme Pharmacist System

Chapter 16 The Perfect PCR Experiment

Wu Yan knows that Zhou Wen must have a special extraction technique.

However, she didn’t want to say that when she saw him, she didn’t force it. After all, this is someone else’s housekeeping skill, and it is harmful and useless to leak out.

Then the two people talked about the salary again. Wu Yan told Zhou Wen what she had said to Shi Lei again, and finally smiled and asked, “Do you have any questions?”

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Zhou Wen thought of the task and said: “Could you ask, am I a contractor or a full-time worker?”

“Well…” Wu Yan hesitated for a while, she didn’t. t expect Zhou Wen will ask this question.

What is the current biological situation? She believes that Zhou Wen should be very clear in her heart that the contract of Number One Person Hospital in Jiang Prefecture is so generous, not to mention that he is a small undergraduate. Many people are also looking for a Master of Biology student and Court Academecian student.

He still wants to ascending to the skies with a single leap, and directly solve the production problem, which is really too much. She herself is still a contract worker.

Wu Yan thought for a while and said: “Generally speaking, if it is hired in the hospital, it must be a formal worker, but you are the employment contract of the PCR team of the testing center, so I am afraid you can only be a contract worker. “

“Contractor…” Zhou Wen hesitated.

Actually, a contract worker is still a formal worker. He really doesn’t care. As long as he can go to the testing center to practice internship, he is willing even if he is a temporary worker.

Isnt this a task that requires to become a formal worker within a week, he cant help but stretch a little.

1000 EXP and 1000 points, there is a chance to open a black iron box.

Wu Yan originally thought that the matter had been done, didnt expect it to be something wrong here, so earnest and well-meant advised and said: “Although you are a contract worker, the salary is calculated according to the regular worker. Yes, not at all. Whats the difference.

Besides, everything comes step by step. Its not too late for you to go to work and wait for a period of time to solve the problem.”

Zhou Wen smiled and asked: “You are my Senior Sister, and you have been in the hospital for a year. To be honest, if I really sign a contract, when will the establishment be resolved?”

“Well…” Wu Yan was a little embarrassed, and she concealed her coffee cup.

After putting down the cup, Wu Yan said: “Well then, I will help you go back and ask. But to be honest, don’t expect too much.”

Zhou Wen nodded , Said with a smile: “Okay, then trouble the Senior Sister.”

“You’re welcome.”

Zhou Wen is still eccentric over there, and didnt wait much, After a few polite words, he got up and left.

_

When returning to the laboratory, Yang Yudong and Gu Hongyun impatient asked him whether the interview was successful?

Zhou Wen laughed and said: “I made a condition, she said she would go back to discuss with the leader, and then give me the letter after the discussion.”

Yang Yudong speechless saying: “The conditions are so good, do you have to mention them?”

Zhou Wen said: “I dont want to see the rare opportunity, so I want to try their reserve price.”

Yang Yudong: “…”

He really wants to use the extraction gun to take out Zhou Wen’s brain a little, and see what he is thinking all day long?

Zhou Wen chuckled went to do a PCR experiment.

The full name of PCR is Polymerase Chain Reaction.

The Chinese name is “polymerase chain reaction”, also known as “polymerase chain reaction”, which is an in vitro nucleic acid amplification technology developed in the mid-1980s.

The target gene or a certain DNA fragment to be studied can be amplified to hundreds of thousands or even millions of times in a few hours in a test tube.

The principle is basically similar to DNA replication in human cells, but the reaction system is relatively simple.

Of course, this does not mean that PCR is simple.

On the contrary, PCR is very difficult.

First, through high temperature “denaturation”, a piece of double-stranded DNA is melted into two single-stranded DNA.

After “annealing”, at low temperature, the “primer” and the DNA as the template are complementarily combined to form a partial double strand.

Finally, “extend” in the middle of the fire. The DNA polymerase catalyzes the extension reaction of the DNA strand with the primer as the starting point to become two double-stranded DNA.

Repeat this process again and again.

1 becomes 2, 2 becomes 4, 4 becomes 8, 8 becomes 16, 16 becomes 32……

Through PCR technology, you can change from a hair, a drop of blood, or even one A sufficient amount of DNA is amplified from the cells for analysis, research, detection and identification.

Such as paternity test;

Such as vaccine research;

Such as virus disease prevention and control;

Or genetic disease research, etc. A lot of PCR is needed.

Of course, Zhou Wen did not need to do PCR in the rice aphid biochemical reagent experiment that Zhou Wen did today.

Aphids are a very low-level insect, generally use medicine directly on it, and then just look at its genetic characteristics.

The reason for doing PCR is to learn PCR more.

The PCR reaction system includes water, buffer, DNTP (deoxyribonucleoside triphosphate), primer P1/P2/DNA template and TAQ enzyme.

After everything is ready, Zhou Wen adjusts the volume of the extraction gun, and then extracts 30_l of water into the three sets of PCR tubes.

Strictly follow the operating procedures when aspirating liquid, aspirate vertically, suck slowly and release slowly.

After changing the tip, I extracted 5 ml of buffer into the PCR tube.

Then add DNTP/P1/P2/DNA template to the tube separately…

Thanks to the learning during this period of time, apart from being nervous at first, Zhou Wen did it Methodical.

After the required ingredients are added, the next step is to centrifuge.

After setting the speed as required, wait 3 minutes for the centrifugation to complete.

Zhou Wen took out the PCR tube containing the mixed solution from the centrifuge, and immediately put it on the PCR machine for amplification.

Set the pre-denaturation at 94 for 4min.

Then set 20 cycles of amplification stage.

High temperature, low temperature, medium temperature, high temperature……

1 change 2, 2 change 4, 4 change 8, 8 change 16, 16 change 32……

Zhou Wen waited anxiously, feeling like a husband outside the delivery room, silently praying for “Mother and child safety”, and at the same time, wondering if the one who will be born is a boy or a girl? Are you healthy?

At this moment, Zhou Wen suddenly thought that system integration could speed up the progress of the experiment. Instead of waiting here for a few hours, how about speeding up?

No one sees it here anyway.

Thinking of this, Zhou Wen immediately summoned out his personal attribute page. After clicking on the points, an option popped up.

[Is it possible to use points to speed up the progress of the experiment? Yes/No]

[Yes].

[This experiment requires 20 points to speed up, please confirm again]

Zhou Wen see that only 20 points can speed up the progress of the experiment, so why hesitate Yes, I immediately chose to confirm.

Before Zhou Wen can react, the PCR machine has completed the amplification in the next second.

Zhou Wen was surprised that the power of system is simply unfathomable.

He sighed in his heart, and soon he became nervous again.

I did PCR for the first time, I wonder if it can be successful?

Zhou Wen turned on the PCR machine and took out the PCR tube from it.

Originally, the following is the product analysis, but Zhou Wen has real vision, slightly squinted, and soon he saw the high-density double-helical structure of DNA.

“perfect~”

If it weren’t for wearing a mask, Zhou Wen would have laughed heartily.

There is each one of the students. Who didnt learn to do PCR after countless failures? Who can do PCR for the first time like him?

And the amplification effect is unexpectedly good, simply perfect.

_

Jiang Prefecture City, Number One Person Civil Hospital, Testing Center, PCR Group.

Wu Yan had lunch outside before returning to the hospital, and then immediately reported to the team leaders office.

“What about people?” Zhang Shuguang, who was writing the experiment report, asked without raising his head.

Wu Yan said somewhat sorry: “That… hasn’t come yet.”

When she went, she patted her chest and promised that it was very easy, and the cowhide was blown up. .

Zhang Shuguang raised his head and said unexpectedly: “Why, I don’t want to come?”

Wu Yan shook her head and said, “No, but he said…”

Yan told Zhou Wen’s request to Zhang Shuguang.

Zhang Shuguang took the pen with his left hand, tapped on the desk, and said with a smile: “You said you, such a smart person, why do you lose your chain at a critical time?”

Wu Yan said strangely: “Why did I drop the chain? He wants a formal salary. How can I dare to overtake it for you, and promise it for you?”

Zhang Shuguang said with a smile: “What’s wrong with the overtaking? , He is a biology undergraduate, dare to ask lion’s big mouth to ask you for a career plan, dont you think about why?”

“Why?” Wu Yan was puzzled, and quickly responded Coming over, he said in surprise: “Oh~~~ You mean…”

Zhang Shuguang said with a laugh: “You have all come to find someone, as long as it is not too outrageous, you should agree to him first. , If you have any questions, Ill talk about it later. The verbal promise is not a pack of votes, right?”

“Hmm~ Boss, I know it was wrong.” Wu Yan repeatedly nodded, admitting the mistake,” Then I call him now?”

Zhang Shuguang shook his head amusedly, “I’m back all the time, what a rush, I’m afraid that he won’t be able to run. Anyway, we are also the Jiang Prefecture First Hospital, so we can’t let it too much. People look down on him, stretch him for two days before talking.”

(End of this chapter)