Supreme Pharmacist System

After exchanging greetings, Zheng Lina half-jokingly half-seriously said that she proposed to work here with Zhou Wen.

Zhou Wen was a little surprised.

He was about to recruit another employee, but he didn't expect it to be delivered to his door.

Originally, Tang Xin was an assistant, which was almost enough, but he has been busy recently, so he has no time to extract high-concentration DNA.

So he planned to train a successor to help him produce high-concentration DNA.

Tang Xin was naturally the best candidate. She knew the basics and had a relatively calm personality, so she was very suitable.

Zhou Wen happily agreed to Zheng Lina's request to stay.

"I'll let Tang Xin teach you the specific work content later."

Zheng Lina was naturally overjoyed, and nodded again and again, "Yeah, I know~ Thank you."

Zhou Wen then said to Tang Xin, "Today I will teach you how to extract DNA."

"Ah... I'm afraid I won't be able to learn."

Tang Xin was a little surprised when she heard this. Although she had dissected mice in college, she mainly did pharmacological experiments and never extracted DNA.

"Hehe, it's very simple, not as complicated as you think."

Just do it, Zhou Wen immediately started on-site teaching.

I took a small white mouse out of the cage, came to the side of the operation table and said: "First of all, kill the small white mouse. This is a little different from your pharmacological tests. We mainly take the thigh bone marrow of the small white mouse..."

How did Zhou Wen's professor Tang Xin extract more mouse bone marrow.

His lectures are very slow and detailed. These experiences are obtained from the theory and practice after he obtained the system, which is simpler and easier to understand than any professor in the world.

Tang Xin can basically understand it once.

Of course, the extraction of DNA does not rely on theoretical experience, without it, it is only a matter of familiarity.

So after Tang Xin understood the principle, Zhou Wen directly gave up the console and let her do it herself, while he corrected the technique on the spot.

Including the centrifugation time, the method of extracting the supernatant, the number of times of pipetting, the amount of fixative added, etc., all are carefully taught.

And this kind of hand-in-hand teaching treatment, even Qin Haitao didn't get it...

For the next period of time, Zhou Wen basically spent all his time in the Institute of Drug Research.

While teaching Tang Xin the method of extracting DNA, he also did pharmaceutical tasks by the way.

In addition, he pumped the air conditioner to prepare some low-osmotic fluids with optimal ratios.

It is the key to ensure that the highest concentration of DNA can be extracted every time, or else only DNA with a slightly higher concentration than ordinary DNA can be extracted.

A week later, Tang Xin was able to complete the DNA extraction independently. Although the concentration is not high.

But it is also a very remarkable thing.

Tang Xin was overjoyed when she saw the mouse DNA under the electron microscope.

"Wow~ that's great..."

...

...

Saturday, December 29, the day before New Year's Day.

The Prion Institute of Jiangzhou First People's Hospital was officially established.

This is also the second prion research institute in China, and its scientific research strength is recognized as the first in China.

The first director of the institute was Li Wenshuo.

Li Wenshuo, whose daughter-in-law has become a mother-in-law for many years, couldn't help getting wet at the unveiling ceremony.

He has been researching prions since his second year of research, and it has been a full eight years now, and nothing has been achieved.

At the worst time, because I couldn't apply for scientific research funding, I had to set up a street stall with a few colleagues.

Thinking about it now, it is also a bitter tear.

Therefore,

Li Wenshuo is especially grateful to Zhou Wen for giving him a hand during the darkest moment of his life, and arranging a chair for him on the rocket in the sky. World-renowned prion expert!

After taking a group photo with some leaders and scholars who came to attend the unveiling ceremony, Li Wenshuo invited several fellow scholars who came from afar at home and abroad to go to the office for a detailed discussion.

As soon as everyone entered the office, they noticed that there was a large photo of people hanging on the wall.

"Hey, this person looks familiar."

Li Wenshuo looked at the photo of Zhou Wen on the wall and said with a smile: "Yes, he is the inventor of PsC1, Zhou Wen, my idol."

Li Wenshuo is not only grateful to Zhou Wen now, he even admires this young man who is nearly 10 years younger than him. That's why he got a photo of Zhou Wen and hung it in his office, so he regarded it as a star chaser.

Professor Qin Ziyu, deputy director of the China Prion Research Institute, regretfully said: "He obviously has an outstanding talent in prions, I don't know why he doesn't continue to study in depth?"

Li Wenshuo said with a smile: "About this question, I also asked him.

He said that there are too many diseases in this world, and various viruses and bacteria are threatening human life and health, which often makes him feel powerless.

That's why he gave up the research on prions, but engaged in more advanced genetic research, hoping to decipher the human genetic code one day and cure more diseases. "

Qin Ziyu sighed after hearing the words: "Although I don't agree with him doing this, but anyway, courage is commendable."

Li Wenshuo nodded, followed by a smile and said, "This morning, MIT..."

No. 2333, Jiangzhou Science and Technology Park, Institute of Cryptococcosis neoformans.

The site renovation here, as well as equipment and personnel, etc., are all in place.

Fifteen newly recruited PhDs in cell engineering also had a preliminary understanding of the next research project.

And the result of understanding is that everyone is dumbfounded.

Based on the level of these people, it is simply impossible to clone antigenic serum cells;

Not to mention that the content of antigenic B cells in serum is as low as a terrible number, even if B cells are isolated, the requirements for the sensitivity and specificity of their sequence amplification and determination are very high.

Even if the sequence is finally amplified and the antibody is expressed, the final effect of the antibody is still open to question.

If they really have this level, how can they be reduced to the point where they can't find a job, and they have already gone to European and American countries to earn money.

During lunch, Henry Zhang, the leader of the research group, approached Qin Haitao and asked, "Do you know what the Zhou Institute is planning? Now the work is completely unable to start, and there is even no direction at all."

Qin Haitao smiled and said: "Be safe and don't be impatient~ You should first learn about cryptococcus and the ingredients of antibacterial serum. As for how to develop it, I will explain it in detail when I come to the Zhou Institute."

Henry Zhang said helplessly: "Okay..."

Jinhu District Industrial Park, No. 404, Huanyu Biotechnology Research Institute.

Unlike Zhang Yang and the others, doctoral students like Wang Wei had a very fulfilling time during this period.

According to the requirements of the company, they have a detailed understanding of mainstream biotechnology at home and abroad, such as molecular biology, transgenics, bacterial communities, natural products and plant synthetic biology, clinical bioprocessing, etc.

91 people, three people in a group, thirty people in a large group, learn from each other, communicate with each other, compete with each other, and initially build a good and orderly academic ecological environment.

Such an ecological academic environment is the dream of all biological students in China.

However, building such an academic environment requires very high funding.

In the past week, 210,000 yuan has been spent, and the average is almost 3,000 yuan per person.

You must know that this is only the most basic guarantee for scientific research. If you wait for the opening of the laboratory and all go to the laboratory, the cost will increase by at least 5 times.

1 million a week.

One month is 4 million.

Don't mention anything for a year, and the research funding alone will cost at least 50 million.

This is also the reason why Zhou Wen hesitated before.

The task seems simple, but in fact the real difficulty lies in the later business development.

If the product cannot be developed and launched as soon as possible, the research institute will close down soon.

At two o'clock in the afternoon, in the office of Hengfeng Building, Chen Zhiyuan looked at the thick pile of bills on the desk with a pained expression on his face.

During this period of time, money is spent like running water, office space, office consumables, equipment, equipment, activity funds, etc., every day you open your eyes is money.

He doesn't know how expensive he is if he is not in charge of the family. This is the first time he knows that the pressure from the boss is so great.

The first 50 million funds have all been credited, but more than 3 million have already been spent.

This is "little money".

The real big picture is the equipment purchase, and the bill on the table is the equipment list.

A total of 36 million! 2 million advance payment has been paid.

Just 10 sets of Hitachi High-tech S-3700N, Hitachi High-tech S-4800, Hitachi High-tech SU-70, etc., and different types of electron microscopes, the total price exceeds 20 million RMB.

So scary!

After Chen Zhiyuan checked the bill carefully, he went to the financial office and asked them to pay the final payment to the supplier.

As soon as he came out of the financial room, the phone rang in his pocket.

I took it out and saw that it was an unfamiliar local number.

After connecting, Chen Zhiyuan said: "Hi, hello, I'm Chen Zhiyuan, who are you?"

"Hello, Mr. Chen, I'm Meng Xiang, the general manager of Jiangzhou Tianhai Intellectual Property Agency Co., Ltd. Mr. Zhou's patent application has been approved, and he asked you to come over to hand over some procedures."

Chen Zhiyuan asked strangely, "What patent?"

Meng Xiang: "The production process of sildenafil granules."

"What, sildenafil?" Chen Zhiyuan exclaimed when he heard the words.

Meng Xiang: "Yes! If you have time now, you might as well come to the company."

Chen Zhiyuan hurriedly said: "Okay, I'll go there now, where is the address..."

After hanging up the phone, Chen Zhiyuan was so excited that he almost jumped up.

He has eaten that powerful sildenafil granule, and the effect is amazing. If it is produced in the future, hehehe...

And Zhou Wen said that this medicine will be represented by Huanyu.

Thinking of this, Chen Zhiyuan immediately left for Tianhai Company.

When he arrived at the company, Zhou Wen followed him.

Then Meng Xiang's words made the two excited hearts go cold instantly, as if a basin of cold water had been poured on them.

According to the Food and Drug Administration, the new sildenafil granules already belong to the category of new drugs. If you want to produce and sell them, you need to go through the clinical trial process again.

Zhou Wen's dream of collecting patent fees was suddenly shattered.

According to the normal clinical trial process, it takes at least 3 years from human trials to marketing.

Chen Zhiyuan was speechless: "Does it take so long?"

Meng Xiang nodded and said: "Yes! The normal process is like this."

Zhou Wen asked, "What about the abnormal process?"

Meng Xiang said with a smile: "There is another way to go through the green channel of rapid review. If it works, the clinical trial can be completed within about 12 months.

Because after all, sildenafil is also a drug that has been tested by the market for many years. On the basis of the same raw materials and formula, only the production process has been improved, so there should be room for operation.

You can talk to the CRO company about this, they should have a solution. "

Hearing Meng Xiang's words, Zhou Wen's eyes lit up immediately, "That's right, how could I have forgotten them."

The so-called CRO, its full name is called "Contract Research Organization" in Chinese.

It originated in the United States in the early 1980s.

It is a commercial organization that provides professional services to pharmaceutical companies, medical institutions, and small and medium-sized pharmaceutical and medical device R\u0026D companies through contracts.

There are three types of industrial chains.

From 0- (preclinical candidate compound) discovery.

From-IND (New Drug Clinical Application) verification process.

Human clinical trial phase of INDNDA (New Drug Application).

Simply put, the purpose of CRO is to improve the efficiency of drug research and development.

You can think of all pharmaceutical companies as e-commerce stores, and CRO is the e-commerce platform.

When it comes to CRO, we have to mention the domestic "Yaoming Pharmatech" and "Tiger Medicine".

For example, Yaoming Kangde is the "Ali of the CRO world". Their slogan is - let the world have no difficult medicines and difficult diseases...