Rebirth of the God-level Xueba

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When Yang Rui chose the correct temperature, the correct length of DNA, and the correct tracer, it was not difficult to make results.

In fact, the work of the vast majority of graduate students is to do known experiments and use known experimental methods to produce results, which is the requirement of the laboratory for scientific research dogs.

It takes three years for a master's degree, and the first year is often dominated by theoretical study and laboratory exercises. At this time, if they do a middle school experiment, they may not be able to produce results.

But in the second year, when graduate students began to do experiments frequently, the speed and success rate of their experiments were greatly improved.

For experiments like the first generation, if a second-year student cannot master them proficiently, he is not qualified to be called a scientific research dog. At best, he is a wild dog, wandering between the laboratory and the laboratory corridor.

Of course, Yang Rui himself has never been in contact with the first generation. When domestic students come into contact with it, it is at least the second generation. It is a very simple and common instrument. Its size and usage are similar to those of a microwave oven. Choose and wait for it to come out.

Compared with the second generation, the difficulty of the first generation is equivalent to that of an oven. Yang Rui has done so many experiments and is familiar with it. But this time, he is more serious about preprocessing the raw materials and giving Raw materials are added with tracers, and the temperature and time are carefully adjusted.

Then, Yang Rui seriously wrote down "" in the experiment book.

However, everyone in the laboratory was serious, and Yang Rui, who was serious, attracted Wei Zhenxue's ridicule instead.

"Yang Rui, it's useless for you to record in the official script in the lab book, you have to do the experiment, right? Oligodeoxynucleotides don't listen to you." Wei Zhenxue strolled to Yang Rui. His current workload is not heavy, and doing experiments is purely a personal hobby, and he has published several papers, so he feels very relaxed.

Yang Rui was also in a good mood, and said with a smile: "How do you know that oligonucleotides don't listen to me."

Wei Zhenxue was inexplicably surprised, and suddenly asked in a very low voice: "Can you make the oligonucleotide listen to you?"

After finishing speaking, he looked back alertly, and said again: "What is your qigong called? Molecular qigong?"

Yang Rui was dumbfounded: "How could you think of Qigong?"

"Why can't you think of qigong? Look at you, you can't do it every day. Why do you do it so seriously today? You must use it. Otherwise..." Wei Zhenxue dragged on for a long time, his eyes slanted, and said: "Are you I asked some master for advice, right?"

Yang Rui's expression seemed to be stared at by a dog, and he looked at Wei Zhenxue with anti-rabies eyes.

Wei Zhenxue thought he saw through Yang Rui and said with a smile: "That's right, I figured it out, wait a minute, let me guess who made it for you,

Master Hu? He recently gave a lecture at the Temple of Heaven, but he is very busy...is that Master Zhu? Master Zhu from Dongcheng, right? "

"Old Wei!" Yang Rui had to interrupt him, and looked at Wei Zhenxue with the eyes of the boss: "You have been quite free recently, and you have learned so many things."

"Live and learn." Wei Zhenxue smiled triumphantly.

"Learn qigong and use qigong to do scientific research?"

"Oh, I'm also in the experimental stage, hahaha..."

"Well, that's good too. In the future, you don't need to use the scientific research equipment in the laboratory, such as microscopes. Let's do qigong testing, save a little equipment resources, so that everyone can be more comfortable, and you can use your waste heat. "

When Wei Zhenxue heard that he was not allowed to use the instrument, he became anxious, and said: "What's the use of residual heat, I'm not old yet. I'm too young, that's why I didn't learn Qigong well. I haven't learned Qigong yet, Yang Rui, I still have to use the instrument , using qigong won’t work.”

"You can't do qigong?"

"I can't use qigong now."

"You can't do qigong?"

"Hey, you're right, qigong doesn't work!" Wei Zhenxue adhered to Chinese beliefs and absolutely only chose those that were beneficial.

Yang Rui hurt Wei Zhenxue a few words, and immediately felt that he was invigorating blood and removing blood stasis, and his whole body was at ease.

Sure enough, there must be one person who is scolded in the laboratory to be harmonious.

After driving Wei Zhenxue to the side, Yang Rui started to simplify the system "-128".

Compared with experiments numbered before 50, the reaction volume of -128 is also reduced, and the reaction concentration is thus further increased.

Yang Rui carefully and carefully recorded all the experimental preparations and experimental materials, and then stared at the water bath in a daze.

At this point, Yang Rui's experiment is slightly different from the original one.

Originally, because there is no heat-resistant polymerase, it takes a few tens of seconds to come in and out of the water bath. When Murris was doing this experiment, he was like the boss of Malatang. Bring it up in a while...

With heat-resistant polymerase, this step can be omitted, and Yang Rui can relax a bit.

He counted the time, put forward something, and started electrophoresis.

The purpose of running electrophoresis is to see the gel of the radioactive tracer.

The gel is like a jelly, and the dna gathered by electrophoresis will form bands on the gel.

If the band is obtained, it proves that there is a product in it, and if the band is not obtained, and the gel is still clean like fresh jelly, then there is no product, which means that the experiment has failed.

Wei Zhenxue quietly stood behind Yang Rui, his knees were slightly bent, his hands were stretched out, his face was as red as constipation.

Yang Rui's attention was all focused on the gel, and he didn't even know that someone was watching behind him.

Yang Rui waited quietly for the completion of the electrophoresis, slowly pulled out the gel, and then placed it under the light of the laboratory table to observe the gel carefully...

"Oh my god... I succeeded, I made a strip!" Wei Zhenxue suddenly shouted like a pig, with his palms facing himself, with an expression of disbelief.

Yang Rui was so frightened that he almost jumped up, turned around and saw that it was Wei Zhenxue, and couldn't help cursing angrily, "What's your name!"

"I gave you the power just now, but you made strips. You see, you see, the color of the strips is a bit lighter, but you made the strips. I did the work, I did the work. !” Wei Zhenxue kept pointing at himself.

"Did you really make the strips?" Huang Mao pushed Wei Zhenxue away, looked down at the gel, and said, "So, your more than one hundred experiments are considered to be over the top."

Not only Huang Mao, but everyone nodded with lingering fear.

Repeating the experiment more than a hundred times is a cruel act, and researchers with weaker psychological endurance will collapse. Seeing that Yang Rui finally made something, everyone breathed a sigh of relief.

Yang Rui also breathed a sigh of relief.

Although the correct experimental method is used, there are still some results that cannot be obtained after reading the experimental manual.

"Let's see, it's really done. I copied the same DNA. Is that what you mean?" Li Wenqiang moved closer and looked at the gel carefully.

"I did my best just now." Wei Zhenxue also moved closer.

Li Wenqiang gently pushed him away and said, "Don't make trouble."

"I've been practicing for half a year." Wei Zhenxue found Zhang Xuetong who came to join in the fun.

"Good boy." Zhang Xuetong patted Wei Zhenxue, turned a corner, and went to see the gel made by Yang Rui.

"There are indeed strips, it can be seen, it is indeed made." Li Wenqiang nodded while talking, his expression did not change much from usual. After all, in his opinion, this should be a good paper, but that's all.

Yang Rui re-examined the experiment, and after confirming it, he called Wang Lei over without hesitation, and said, "Call Li Zhangzhen and ask him to find a patent lawyer, as soon as possible! You need a high-level barrister, don't If you are afraid of spending money, it is best to go to a large law firm."

"What lawyer?" Comrade Wang Lei, the new professional experiment dog, still doesn't know these words.

"Patent lawyer, call me first if you don't understand." Yang Rui said and said: "I will do another repeated experiment, Zhou Yuwen, go and take out the camera in the cabinet, and give me the video from beginning to end. , don’t miss a little detail, use the video recorder, you must pay attention when the tape is almost finished, you can notify me in advance.”

Don't worry about leaks, this technology is invaluable because it is easy to use and widely spread.

Compared with keeping secrets, Yang Rui cares more about patents and honor attribution.

Between the two, Yang Rui must first determine the honor. Video recording is the most commonly used method for recording experiments in later generations. It is very convenient, simple and sure to determine the ownership of the results.

There were no digital cameras in 1984, but Yang Rui didn't care about wasting video tapes either.

Huang Mao knew that Yang Rui often had a target, so he thought about it curiously for a while, but he couldn't figure out why.

The newcomer Li Wentong and others somewhat felt that Yang Rui was making a fuss out of a molehill, but the advantage of a private laboratory was that the boss was very authoritative. Yang Rui used his own money to do things, and others had nothing to say.

...