Rebirth of the God-level Xueba

Yang Rui first quickly browsed Haizhong's papers to make sure that he would not say more, say less, or even say something wrong.

The development of the biological field is very fast. Unlike other disciplines, the rapid development of the biological field is not only a forward change, a horizontal change, but also a change in the concept itself.

In other words, the same word, which has one meaning now, may have another meaning in the blink of an eye.

How fast is it? If a Ph.D. in the Department of Biology pursues romance, he accidentally creates an explosion when he proposes to the goddess. He is imprisoned for 5 years because of accidental death. When he comes out again, the child of the goddess happens to be in the third grade ——This is not the changing part, this is a normal situation, the changing part is that this doctor of biology is now teaching biology to the child of the goddess, his knowledge is outdated and outdated, he has to make up for himself first.

The world is changing so fast.

To make a comparison, the Eulerian geometry of mathematics was invented more than a thousand years ago. Newton had five axioms when he was in junior high school, and Lu Xun had the same five axioms when he was in junior high school. In the 21st century, Riemann corrected the last one. As a result, it was too difficult, not to mention that junior high school students did not learn it, and mathematics undergraduates did not necessarily learn it.

Physics, chemistry, even metallurgy, and architecture are commonly used knowledge that was thousands of years ago, or at least hundreds of years ago.

Only biology, the Nobel Prize will be awarded this year, and the teaching materials will be changed next year.

However, if you use those advanced vocabulary before mainstream academics recognize it, the situation will become very interesting.

This two-way selection meeting carefully prepared by Professor Cai is likely to turn into a huge discussion field, which completely deviates from the original purpose.

Yang Rui thought about it for about a quarter of an hour before he said carefully: "Actually, I published a few papers before working on the potassium channel, and when I was working on the potassium channel project, I also did a few small experiments. , is not written in this paper because it is irrelevant, but it is indeed a very good subject."

"Oh? What is it?" Professor Cai was in charge of presiding over the meeting, and when Yang Rui mentioned the potassium channel, he nodded slightly.

It is the most correct way to start with the potassium channel.

Yang Rui sat up straight and said, "It's the study of proteins in potassium channels, but it's not the current mainstream g protein, but the interaction between proteins."

"Go on."

"Protein is the basic functional unit of life activities,

All changes in physiological responses are inseparable from the interaction between proteins. When I was working on the potassium channel project, I discovered that we not only need to understand the structure and function of a single protein, but also need to understand the functional process of the protein's internal action..." At this point, Yang Rui paused and explained: "Repeat potassium The study of the structure of the channel may be a simple and clear choice, but just to do the structure of the potassium channel will soon have nowhere to go, and the level of papers that can be published will also decrease step by step, but the relationship between proteins The relationship is different. I did not find relevant research results in an experiment I did before, but because of time, I haven't done a careful comparison and formed a paper. "

In the last sentence, Yang Rui was just making up nonsense, he just found some papers. However, interacting proteins are an important correlation study of potassium channels. Yang Rui did read a lot when he was working on the potassium channel project.

Regarding the interacting protein, Professor Cai was noncommittal, but grasped a point in Yang Rui's words, and asked, "So, the laboratory you lead won't be doing projects on the structure of potassium channels?"

"It's not impossible to do it at all. If the funds are sufficient, you can do some in advance." The biggest difference between leading a laboratory and joining a laboratory is that one is a boss, and coordination and command are more important, and the other is a worker, who must participate in the field .

The boss can have multiple laboratories at the same time, and the migrant workers usually can only work in one laboratory.

For Yang Rui, if someone is willing to write top-level papers in his laboratory, he has no reason to object.

As for the guidance, it didn't take much time. When he returned home during the Chinese New Year, Huang Mao was still lying in the laboratory doing experiments, and the progress was not affected too much.

Good guidance is like poking the window paper. The problem is not how strong it is, but the direction and distance.

Most of the laboratory leaders are only conjectures for the guidance of papers. The purpose of raising a large group of experimental dogs is to let them realize their conjectures. Sometimes it is an ingenious method, and sometimes it can only be stupid trial and error. up.

However, Yang Rui has done a batch of potassium channel projects, and it is very easy to instruct again. Although it is not as easy as a school chemistry teacher instructing students to do standard experiments, it is not much more difficult than walking a dog.

And Yang Rui's answer was also recognized by everyone, and many people showed satisfaction.

Yang Rui's answer shows that this student has a clear mind, not only a clear IQ, but also a clear EQ.

This is a very important point for professors and associate professors who are not familiar with Rui Yang.

The only reason why Yang Rui can sit here is that there is an article called "Cell". If he breaks away from the Potassium channel, breaks away from his first achievement, and completely starts over, he will be a rootless duck. Few people will Build a lab with him.

So far, all the people present have considered co-construction of the laboratory, and often put Yang Rui in this position.

From everyone's point of view, this is already an excellent treatment.

As for the ** laboratory that Professor Cai mentioned, it is more like a picture cake. It is far more difficult for Yang Rui to get it than to build a joint laboratory.

Even Professor Cai himself was hesitant.

In fact, more than half of the scholars here are inferior to Yang Rui in terms of achievements and papers.

The three journals "Cell", "Nature" and "Science" are the bibles in the field of scientific research. They are the hardest conditions. The whole of China can't publish fifty articles a year. If they are assigned to the field of biology, they can't meet them. Ten articles.

Although the number of papers with this number basically falls under the names of top universities or institutions such as the Chinese Academy of Sciences, Peking University or Tsinghua University, after so many years of accumulation, most professors still do not have the opportunity or ability to publish papers on CNS.

For example, Professor Tang Zhong, although he has published many papers in syst- (systems biology) with an impact factor exceeding 10.0, but a cell is a cell, and sb will always be two worlds.

Of course, the impact factor of the papers published by Professor Tang Zhong is low, and there are also factors of the times. Before the reform and opening up, most Chinese scholars preferred to publish in Chinese journals, and the Chinese journals at this time were still alive and well.

On the other hand, the current Chinese universities are not based on the theory of only influencing factors.

However, no matter how you look at it, "cell" is also a shocking force. If Yang Rui is not a student but a teacher, even if he is a small teaching assistant who has just graduated from senior year and stayed at the school, he is also qualified to lead a laboratory independently.

Now it's been 3 years, and it's always hard to turn around.

However, Professor Cai still gave Yang Rui a chance.

This is already the best effort he has made without being influenced by tradition, inertia and secularism.

But for now, Professor Cai didn't want to follow Yang Rui's words and asked instead: "You said that the funds are abundant, how much money do you think you need?"

"US$200,000, plus 3 million RMB." Yang Rui's potassium channel project cost more than US$600,000, and it still hurts to think about it now.

Such a project basically won’t get a penny back, and there is nothing to apply for a patent. Even the research based on it is still basic. Only the research based on this research is possible to apply for a patent. technical research.

However, just like there are experimental films, literary films, and commercial films in movies, most of the things that go for awards and styles are pure losses.

There are still very few projects that gain both fame and fortune like this.

If it wasn't for the purpose of accumulating reputation, Yang Rui would not invest in his own dissertation on potassium channels.

In the future, it is better to leave such research to the national laboratory. It is better for national taxation to be ruined by scientific research dogs than by government apes.

Dogs are man's friend.

Professor Cai was not surprised by the number proposed by Yang Rui, and asked, "Is it enough? How long will it take?"

"Depending on the proficiency of the experiment and luck, three months to half a year, of course, more money must be faster."

"If it's US$100,000, what about 4 million RMB?" Professor Cai doesn't think Yang Rui wants more, but he still wants to save a little foreign exchange.

Yang Rui smiled and said "No", and said: "Most of the reagents have to be imported."

"If such a paper can be written in half a year..." Professor Cai pointed to the "cell" on the table and said, "I will approve this funding."

"Thank you, Professor Cai, then I will continue."

"There are not many laboratories in our school with a cost of 200,000 US dollars and 3 million RMB." Professor Cai reminded Yang Rui.

"I understand." Yang Rui knew that if the original project was redone, many associate professors and even professors would be willing to join, because they would have a great chance of getting the honor of the first author or joint first author of CNS.

But for Yang Rui, the nutritional value of such a laboratory is too low. Next year, if you want a new project, you have to have another meeting, which may be more difficult than this time.

After a little deliberation, Yang Rui said again: "Let's talk about the interaction of proteins first. I have learned a little bit about it before. There are probably several methods for detecting the interaction between proteins. I think that after the establishment of the new laboratory, You can start with the detection method first.”

"En." Professor Cai nodded. The detection method is purely technical, relatively stable, and can obtain a certain amount of results.

Yang Rui cleared his throat and said, "What I think can be done, first, protein affinity chromatography; second, affinity blotting; third, co-immunoprecipitation; fourth, glutathione transferase precipitation experiment; Fifth, chemical cross-linking method; sixth, fluorescence resonance energy transfer method..."

When it came to this, the professors who were listening to Yang Rui with a smile on their faces, slowly became serious.

It's just a list of detection methods, many people present can do it, it's nothing more than memorization or reciting, not to mention some powerful people who can remember it with a photographic memory.

However, Yang Rui's proficient list shows that at least he didn't just talk about it.

Under the attention of everyone, Yang Rui said three more without stage fright: "Seventh, surface plasmon resonance technology; eighth, phage display technology; ninth, yeast two-hybrid or multi-hybrid..."

When it came to the seventh, many people, including Professor Fu, became unnatural. The reason is very simple. They have heard the term of surface plasmon resonance, but they don't understand the specific content.

\u003cCalpa\u003eProfessor Fu immediately objected, saying: "What you just said is that the new laboratory can do it. Can you do these tests?"

Detection methods are not just detection methods. Newer and more advanced detection methods often represent more accurate and fresher conclusions. In practice, it means more and better papers.

This is much more meaningful than the meaning of ethereal protein.

When Yang Rui was studying, these detection methods were not new. They belonged to common laboratories. He did not talk nonsense and said directly: "Surface plasmon resonance technology is based on the detection of the spr angle, which is the resonance angle, during the biological reaction process. The dynamic changes of biomolecular interactions to obtain specific signals of biomolecular interactions. It is necessary to apply a computer and add software to analyze the ligands and molecules to be detected on the chip to detect antigens and antibodies, receptors and ligands, proteins and nucleic acids, proteins and small molecules The interaction between them is also very convenient.”

Professor Fu opened his mouth, unable to refute. Because he didn't understand either, he could only look around as if asking for help.

Lu Yueping, who once recommended Xu Anqing to Richard, couldn't see it, coughed, and said: "The principle of surface plasmon resonance technology is right, I used it when I visited the UK, but the computer technology used is still relatively complicated. Yes, can you do it?"

"I have used a similar computer before, and it exists in Huarui's laboratory. If you are interested, I can demonstrate it to you on the spot." Yang Rui didn't say a word. Although the current computer is complicated, Yang Rui doesn't find it very difficult to learn. , In fact, the academic software of later generations is not simple. The wonderful human-computer interaction and friendly interface have never belonged to scientific research dogs.

Everyone was in awe. Nowadays, just using a computer to do experiments is already very academic.

Professor Cai was also slightly moved. Yang Rui's youth seemed to be an advantage at this time.

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