Rebirth of the God-level Xueba

In the laboratory, Yang Rui scratched his head and wrote a note on the paper, thinking about the next experimental goal, seemingly leisurely, but in fact his brain was running violently.

On the contrary, Huang Mao and others were working conscientiously in front of the test bench, busy re-verifying the data to complete the minor revision of the paper.

The dynamic and static relationship between the experimental dog and the researcher is almost exactly the same as the human-dog relationship in the park throwing a frisbee and walking the dog.

If a cynical lab dog saw this familiar scene, it would certainly be talking about lab exploitation.

However, this is how a normal laboratory works.

And the more high-end the laboratory, the more meaningless the simple toil and hard work.

As the person in charge of the laboratory, Yang Rui's work in the laboratory is mainly to ask questions.

The question mentioned here is actually to put forward one's own hypothesis or idea by synthesizing existing scientific research results, especially the hotspots at the frontier of science.

For example, in the study of potassium channels, the hypothesis put forward by Yang Rui is that the structure and properties of ion channels can be verified by cloning mutant genes.

This can be said to be a very difficult and wide-ranging hypothesis. In order to realize it, Yang Rui proposed a second-level idea: we can target the gene we want to mutate.

Similarly, in the second layer of ideas, Yang Rui has further refined ideas, for example: targeted mutagenesis organisms, preferably fruit flies.

This step-by-step conception and hypothesis seems easy, but it is actually difficult, and it is also the first condition for becoming a laboratory leader.

The work of Huang Mao and others is actually to solve problems and complete Yang Rui's ideas and assumptions.

In order to complete their own work, Huang Mao and others have to put forward their own hypotheses, which is also their self-training. The better researchers they are, the faster they can come out.

As far as natural science is concerned, assumptions and hypotheses must be based on a solid foundation, that is, pre-research results already exist.

Of course, how firm it is and how many options it has depends on the level of the proposer.

If you are at a low level, you should obediently stand on a solid foundation, do the work that can be done with your hands, and give human civilization an untraceable force forward.

The higher the level, the lower the requirements for the pre-research results.

Classic examples, such as the Manhattan Project to build the atomic bomb, at the level of Oppenheimer at the time, he was not qualified to put forward this idea and hypothesis, and Einstein was the one who led the letter to Roosevelt, and it had to be Einstein. Start such a project with nothing, not even a little experimental proof.

In fact, it was scientists from Europe who promoted this project, and they persuaded Einstein to have the letter to Roosevelt.

Otherwise, according to the normal research model, the Manhattan Project can wait for 20 years of pre-research, at least until someone makes enriched uranium before there is a basis for making nuclear bombs.

But because of the state of war, and because of Einstein's appearance, the prerequisite requirements of the Manhattan Project became lower.

Instead the project got bigger.

What Yang Rui is thinking about is how big and how big his new project can be.

What he wants to do most now is technology.

According to the timeline, the original author, Mulis, should start to consider related ideas now.

But Murris was notoriously a slacker, or, in a better way, free-spirited.

Therefore, although there are related ideas and the development of the project is not difficult, Muric still spent more than two years, and it was only partially completed around 1986, and it took until 1987 to fully complete it.

It would be safer to do it now. However, Yang Rui only has an unpublished "Cell", and he will enter it immediately. It will be no problem to make it, but he is not sure whether he will get the corresponding reputation.

Although the scientific community is a place where results speak for themselves, the scientific community is a collection of people after all. There are not a few researchers who have received too much praise, or have not received the rewards they deserve. Some of them, in the next In the past few years, they have been recognized and even compensated, but some people may be completely buried in the pile of old papers.

Murris himself is really lazy enough, but the company behind him is not like this. On the contrary, by next year, after the company has confirmed the certainty, it has invested a lot of resources, including many excellent researchers for Murris. deputy.

After much deliberation, Yang Rui decided to seize a favorable position first.

Although I don't have the corresponding prestige, I probably won't get the Nobel Prize—but, who is sure about getting the Nobel Prize?

Einstein almost missed the Nobel Prize because the theory of relativity had not yet been confirmed, and finally had to use the photoelectric effect to award the prize.

With a small body like Yang Rui, or in other words, a small body like Murris, whether he can win the award really depends on his fate.

Since it depends on fate, there is no point in suffering.

Thinking of this, Yang Rui began to slowly write down a few key points on the paper.

It is also a project, but Yang Rui is not going to repeat the steps that Murris did back then. To be precise, he cannot repeat the steps that Murris did back then.

Because in the final analysis, it is just a layer of window paper, which is easy to pierce but not easy to tear off.

Yang Rui didn't want to poke a hole and attract a pack of wolves.

Although it is likely to fall into scientific research competition again, from Yang Rui's point of view, he still hopes that the later the scientific research competition takes place, the better.

He doesn't have the backing of a billion-dollar biotech company.

The most ideal way is that when Yang Rui has established an unrivaled advantage, the later competitors will not have large laboratories to participate in the competition due to cost-benefit considerations. As for those scientific research apes who are always staring at hot spots and doing experiments, they cannot be eradicated. These scientific research apes are all scavenger, but they do not affect the work of the main laboratory.

"Then, let's start with the heat-resistant polymerase." Yang Rui focused on the last step.

Although it is the last step, heat-resistant polymerase is the pre-technology of practical technology and a necessary material. Making it in advance can speed up subsequent research and development and reduce costs.

On the other hand, this is a very critical technology, and as early as 1973, there was related research.

Before the technology came out, the technology was not taken seriously, because people also knew what it could be used for, and the researchers themselves did it out of interest. In 1973, a female student from Taiwan, at the request of her instructor, took The thermophilic bacteria found in the hot springs of Yellowstone Park were used as the research object, and the high-temperature-resistant taqDNA polymerase of the bacteria was successfully isolated. Yang Rui didn't even know whether she had applied for a patent.

At the time, this may have seemed unnecessary, and no one could have imagined that this would end up being a key part of a $300 million technology.

However, even if the other party has not registered a patent, but has a paper in hand, it is not allowed to rush to register. Yang Rui's brains turned quickly, and he thought of a new method in an instant: find a new thermophilic bacterium as the research object, use a new method, Isolation of thermostable polymerases.

Two researchers can independently apply for a patent if they complete their respective research independently, which is also a conventional means of bypassing patent barriers.

Yang Rui then searched for relevant papers in his mind.

Thermophilic bacteria are microorganisms that live in high temperatures. There are many types of them. There are also specialized researchers and laboratories to do related research. If Yang Rui devotes himself to them without preparation, he may not be able to get out after playing for a lifetime.

He had to find a well-studied thermophilic bacterium, confirm that it can be used for the isolation of high-temperature polymerases, and then start related projects.

As far as the current scale of Sinovel Labs is concerned, it is not qualified to do wild and money-burning trial and error methods like top labs.

Ten minutes later, Yang Rui scribbled down the word "Tengchong" in his notes.

The writing was dark and bold.

The thermophilic anaerobic bacteria in Tengchong Hot Spring is the first extreme thermophilic bacteria discovered in China. After it was discovered in 1998, Chinese scientists performed whole-genome DNA sequencing, and thus became the first Chinese to decipher the genetic code. microorganism.

In the biological world of China, Tengchong Thermophile is a famous bacterium. Yang Rui has various studies on this microorganism in his mind, such as archaeal chromosomal proteins, new mechanism of bacterial spore formation, and isolated extremophilic enzymes...

It is a natural way to isolate thermostable polymerases.

"Let's go to the hot spring." Yang Rui suddenly raised his head, his eyes shining brightly.

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