Rebirth of the God-level Xueba

Yang Rui drove the car from the vicinity of Shichahai to the laboratory. It took only 20 to 30 minutes. In the capital without traffic jams, the young people in the future may be... Well, they can feel it during the Spring Festival.

The researchers in the laboratory did not have Yang Rui's relaxed attitude.

Wang Liangcai, Su Fan, Gu Qiang, Tian Bing, and several auxiliary researchers who were transferred were all a little uneasy, either with solemn expressions, solemn expressions, or heavy weight...

Judging from the experimental results, they feel that they have made a breakthrough, and they have already carried out repeated experiments to verify that they have to be sure and sure to pull the lab boss back from the bed so late.

The reason why the team is ready is to gather people and conduct repeated experiments to verify.

Of course, the main reason is that everyone didn't get off work.

The food and drinks in the laboratory are delicious, and no matter how late you stay, there will be a chef to help make supper, and you can also pack the leftovers home, and there is also overtime pay, who is fine and goes home.

Besides, what can a lab dog do after get off work? Do you want to chat with your wife?

Just kidding, can lab dogs have wives?

Does the wife of the experimental dog like to chat? If she liked to chat, why would she marry a taciturn lab dog?

All of the above points to one point.

A group of extremely boring guys, although they have spent many hours in the laboratory, they are still not convinced that they have really achieved a breakthrough.

The project they are doing is "cloning", and it is cloning mammals.

Such a project may casually produce dozens of top-level papers. It is no exaggeration to say that if the sheep-cloning project concentrates on publishing papers, half of the pages from "Cell" to "Nature" and then to "Science" must be set aside for preparation.

In other words, if the immediate results are confirmed, the research team has booked at least one S.

"Wang Liangcai, your team did it? What's the situation, tell me directly." Yang Rui walked in and glanced at it, hung up his coat, and talked while walking.

Wang Liangcai coughed a little nervously, and said: "Yes, we have completed the step of injecting nuclei. So far, the indicators of the injected cells have met the requirements. Some indicators have exceeded expectations."

"Our team also completed an injection." Gu Qiang cut in time, and interjected: "Also, our team completed it a little earlier than Wang Liangcai's team."

Yang Rui turned to look at Gu Qiang and asked, "Why didn't you call me? Lost your number?"

"How can it be, I have memorized it all by heart." Gu Qiang said in a slightly lower voice, and said: "We want to call you after we have done a repeat experiment."

"Is it ready?" Yang Rui asked.

"Not...not yet." Gu Qiang replied with some helplessness. This is not the first time his team has completed the injection process. In fact, his team was the first to complete the injection process.

However, difficulties are easy to break through, and experience is rare.

Continuously completing the nuclear injection does not mean that they will be able to obtain the nuclear injection egg cells that meet the requirements, and even if they get the nuclear injection egg cells that meet the requirements, it does not mean that they will be able to repeat them smoothly.

It is difficult to repeat the experiment, which is a big trouble for Gu Qiang's group.

He always relied on luck to overcome difficulties again and again.

However, repeated experiments can't just be done by yourself, but also have to be done by others.

Only in this way can a helper be obtained to ensure a stable supply of nucleated oocytes.

In order to complete the cloning of sheep, a large number of nucleated egg cells are needed.

Yang Rui's plan is to prepare 1,000 copies of nucleated oocytes, because in history, the Scots used about 550 copies of qualified oocytes to obtain a cloned sheep, Dolly, the only cloned sheep, and the process of pregnancy It was quite dangerous, and several sheep companions were lost in the circle.

Now, Yang Rui has carried out the sheep cloning project several years ahead of schedule, and the conditions in China are weaker than those in the UK. Caring for ewes is a technical job, and the oocytes that have been abused are even more congenitally deficient...

therefore,

When Gu Qiang's experimental group could not repeat the experiment stably, it was not enough to get only one nucleated cell that did not break down, even if it got a nucleated cell that reached the target.

Yang Rui could guess that there was a problem with Gu Qiang's group without asking carefully.

However, he came here today not to be Gu Qiang's bosom brother.

Yang Rui just nodded slightly at Guqiang, then turned to Wang Liangcai, and said, "Give me the experiment record, let's start talking."

Yang Rui reached out to get the record, and turned his fingers while looking at it, signaling to Wang Liangcai to proceed simultaneously.

Wang Liangcai took a deep breath and said, "We have improved the method of enucleation."

"Oh? Second improvement? Not bad." Yang Rui's attention was instantly attracted.

The method he gave was already very advanced, which made him eager to hear Wang Liangcai's improvement method.

"I'm talking about the main part." Wang Liangcai said: "Before, we used the spindle to hold the enucleation needle, and then sucked out the fragmented nuclei."

"Yeah."

"The improved method still uses the tougher properties of the spindle, but we suck in a part of the spindle directly."

"Oh?"

"The advantage of doing this is that it is possible to completely suck out the nucleus, with less pollution and faster speed." Wang Liangcai paused, and said: "Although the success rate is also reduced, I think it is worth it."

Of course it is worth it. Yang Rui greatly admired Wang Liangcai's experimental talent, and even felt a little... a little jealous.

The so-called experimental genius is like this.

In order not to be too advanced, Yang Rui actually did not give the most perfect solution, but only gave the basic version of the solution, simply using the spindle to make a fuss.

Who would have thought that Wang Liang had just learned the basic version, and he advanced it himself.

This kind of acumen and execution is really breathtaking.

Yang Rui originally planned to explain the improvement of the experimental method when the large-scale nuclear cell injection was carried out-it was only the experimental stage, and there would not be much delay, and it also had the benefit of step-by-step practice.

Who would have thought that Wang Liangcai solved the mystery just like that.

Yang Rui nodded helplessly and agreed, and said, "The idea is very creative, what's the result?"

"Comparative experiments prove that there is significance." Significance refers to the data, which Wang Liangcai omitted at this time. To laymen, it seems to be a bit of slang, but there is no violation in the laboratory.

The experimental results are naturally significant. Yang Rui smiled secretly, turned to the part of the experiment record, and checked it.

Although it was the expected result, he still needed to verify it carefully.

Academic fraud occurs in the details, and no one will modify the final figures and then make the results match. Of course, there are always idiots, but not prominently.

The normal academic falsification mode starts from the original data, changes the initial number, and then makes the subsequent numbers through normal calculation icons, which looks serious.

Among the responsibilities of the project leader or the director of the laboratory, the most important point is to suppress the impulse of other scholars in the laboratory to fake, and at the same time, bear the consequences of all academic fraud.

Therefore, when the Japanese beauty researcher was still quibbling after making a fake, her mentor resolutely committed suicide.

At this point, the project leader is a bit like the head chef of a western food kitchen. He doesn't have to do it himself, but he has to check everyone's work and do the final quality control work.

Wang Liangcai had confidence in the experimental records and was not worried about it. After Yang Rui looked at it for a while, he continued: "The denuclearization part is just a small modification, not the most important breakthrough."

"I have more confidence in your group." Yang Rui couldn't help showing a smile on his face, which made other researchers heave a sigh of relief.

Wang Liangcai also quietly breathed a sigh of relief.

Wang Liangcai likes Yang Rui's way of speaking and doing things.

He likes that Yang Rui uses "your group" or "your group" to describe. The usual habit of domestic scholars is "your group", and the difference between the two is individualism or collectivism.

In the 1980s, individualism was undoubtedly condemned and oppressed by collectivism. If it were a different person or another laboratory, the words "your group" would be enough to hold a criticism meeting.

However, Yang Rui can say whatever he wants, and those who accept it don't have to worry about bad influence.

Yu Ze, who carried Yang Rui's authority in the words "your group", made Wang Liangcai, as a secondee, able to enjoy himself in the genetic engineering laboratory.

As for Yang Rui's way of doing things, he won the praise of the researchers.

Everyone is so busy that they don't have time to carry on the family line, and you're still holding meetings or something, believe it or not, I'll slap you in the face?

Wang Liangcai smiled, and said again: "In terms of injecting nuclei, we decided to learn from your previous technical experience, using pulses to break through the plasma membrane, and at the same time, using a transparent bag for fixation."

"That is to use a strong pulse." Yang Rui is very familiar with the details of the experiment, and the related research on cloning sheep is really overwhelming.

Seeing that Yang Rui understood, Wang Liangcai said excitedly, "That's right", and said, "We have done a lot of comparative experiments, and finally determined the minimum intensity pulse that can destroy the plasma membrane..."

Yang Rui stopped listening again.

What Wang Liangcai was talking about was a very small point, but a correct fulcrum can lift... at least the earth.

Using pulses to destroy the plasma membrane is a huge change compared to the previous method of injecting nuclei. In this way, some problems will also be encountered, but if it is solved, it is indeed a broad road.

"Professor Yang, what do you think?" Wang Liangcai's soft inquiry awakened Yang Rui from his contemplation.

"I still need to see other people's repeated experiments, but, Lao Xu, we can focus on the activation of the reconstructed embryos and in vitro cultivation for public relations." What Yang Rui said meant that the progress of the project was advanced.

Everyone in the laboratory cheered softly.

Only Gu Qiang walked back to the backyard in a somewhat dispirited manner, staring at the incense burner in front of the courtyard wall, silently in a daze.

I feel that the experiment went smoothly, but why can't Wang Liangcai do it?

No, what I did before was smoother than Wang Liangcai. Which variable has changed? Did the result change?

Gu Qiang stared blankly at the front, wishing to come up with a formula to solve this problem.