Rebirth of the God-level Xueba

"Professor Yang, we want to do a batch of comparative experiments now to increase the success rate." Su Fan and Wang Liangcai prepared for several days before going to Yang Rui.

Although he was working in a laboratory, the person in charge of the laboratory was not easy to disturb casually. He didn't have any dry food in his stomach, so he was easily bullied.

Yang Rui is not pretending to be a know-it-all when he sits in the laboratory.

Even for the sheep cloning project he started, he would not do every experiment personally. In fact, for such a large-scale project, his task is to supervise the completion of key points. As for those small parts, it is not worth investing energy.

Therefore, Yang Rui didn't pay any attention to Su Fan and Wang Liangcai's question, and nodded calmly, saying, "Very good."

Originally, comparative experiments were the only way to go. If Yang Rui had to teach this, the team would be too weak.

Su Fan has long been familiar with Su Fan's character, and said with a smile: "Professor Yang, we have some ideas on comparative experiments, and I would like you to have a look."

"Oh? Which ones?" Yang Rui is still willing to do this specific question.

"The first is the pretreatment solution. Gu Qiang used 3% sucrose, which seems to be very effective, but he can't tell why. Considering that the sucrose solution may cause certain chemical damage to oocytes, we think whether We need to do some research on the ratio of the sucrose solution." Su Fan tentatively asked the first question.

Yang Rui nodded and said, "The idea is correct, what's the problem?"

He has automatically and consciously put himself in the shoes of a problem solver.

As soon as Su Fan heard that there was something interesting, he quickly said: "I wonder if we can ask the laboratory to organize a team, or send us some additional funding and manpower to do this comparative experiment."

"Hmm..." Yang Rui was a little hesitant about continuing to increase resources for Su Fan and Wang Liangcai's team.

Their progress is not slow, but such a steady and steady style is actually not very recognized by Yang Rui.

Given the current economic situation in China, even Yang Rui’s laboratory cannot get the international average level of funding. It can only be said that the lower price level reduces the laboratory’s operating expenses, but many experiments point out that they are all funded in foreign exchange. , Funding is always tight. If every problem is overcome with resources, it will definitely be weak in the later stage.

"It's just a pretreatment solution, and it's a bit of a big deal to start a research group." Yang Rui pondered for a moment and expressed his objection.

Changing to a local university and doing some research on this seems to be a good topic, and many scholars probably don't mind investing a year or two here.

But from Yang Rui's point of view, there are too many steps in cloning sheep, and the pretreatment solution is just a small point that cannot be smaller, so what if the research is thorough.

Su Fan tried his best to keep the expenses to a minimum, and said: "For the pretreatment solution, an additional 100,000 to 200,000 yuan should be enough."

If you save some, 200,000 yuan can still study the pretreatment operating fluid relatively clearly, and 100,000 yuan is just a joke.

Yang Rui shook his head even more.

200,000 yuan is not a lot. Although the total amount of funds he received is in the eight figures, each project and research group is costly. Just a pretreatment operating fluid costs 200,000 yuan. A good example.

In the final analysis, when doing projects in China, the right way is to save as much money as possible.

"I think you can adjust your development direction." Yang Rui thought for a moment, then pointed out: "Your problem is that the sucrose solution will cause chemical damage to the oocytes, so you want to choose a solvent with as little damage as possible. yes?"

"Yes. We hope to increase the success rate as much as possible. The chemical damage caused by sucrose solution is difficult to estimate. We are worried about the impact on the later success rate." Wang Liangcai quickly explained to Yang Rui.

Yang Rui nodded and said, "It's not difficult to understand your concerns, but the degree of chemical damage is difficult to verify.

"

"Yes..." Wang Liangcai felt helpless.

"I think this issue should be considered from two aspects." Yang Rui said slowly.

When it comes to pointing out the direction of experiments, Yang Rui has always been generous and doesn't have too many scruples.

Seeing direction from chaos and driving in fog are originally what scholars do.

If you do well, you will only get praise, not doubt.

Wang Liangcai regained his energy all of a sudden, and listened to Yang Rui's words with bated breath.

Yang Rui considered his tone, and said: "First, let's talk about the pretreatment of the operating fluid first. You can not only consider it from the perspective of reducing chemical damage, but also from the perspective of protecting oocytes."

Wang Liangcai and Su Fan looked at each other without saying a word. This is one of the obvious strategies, but the strategy may not be realized.

Yang Rui tapped the table twice with his fingers, decided to speak more clearly, and said: "If I were to do this experiment, I would definitely not use pure sucrose solution. I will consider giving the oocytes a more suitable environment, such as Said, fetal bovine serum is a good choice."

"Fetal bovine serum..." Wang Liang didn't expect that Yang Rui could really say something, and it sounded quite reasonable.

In the genetics laboratory, serum is also a commonly used reagent, and fetal bovine serum is not unfamiliar to people. However, if Yang Rui hadn't proposed it, Wang Liangcai and Su Fan, who were full of sucrose solution, would not have thought of it.

In fact, including Gu Qiang's group, the entire laboratory has never tried to add fetal bovine serum to the operating solution.

"Let's try it out." Wang Liangcai became more excited.

Judging from experience alone, adding fetal bovine serum is also a very reliable choice.

"Director, what about the second point?" Su Fan also thinks it is reliable. He has been doing cell cloning for a long time, and he also used fetal bovine serum. Looking back now, all the indicators are very consistent.

Yang Rui smiled and said: "Second point, I think you still need to change your thinking."

"Yes."

"Our goal is to clone sheep, um, asexually reproduced cloned sheep... All in all, this project is not yet a commercial project, so we only need to make a cloned sheep in the end. The success rate and cost performance are not high.”

What Yang Rui said seemed very easy, as long as there was only one cloned sheep, Wang Liangcai and Su Fan felt helpless.

However, what Yang Rui said made sense. If the funds are sufficient and the manpower is sufficient, the bottom-up push is naturally the simplest and most feasible model.

However, it is basically impossible to have sufficient funds and manpower.

There are very few scientific research projects that allow scholars to consume resources wantonly.

From Yang Rui's point of view, sheep cloning is a bottomless pit project.

Even after another 30 to 40 years, people still have only a half-knowledge of the various key points in cloning.

Historically, the Scots did more than 600 experiments before making a cloned sheep called Dolly. If their preliminary research is sufficient, even if it is an insurance measure, two or three cloned sheep may eventually be born.

It can only be said that the appearance of Dolly the cloned sheep also has a certain element of luck.

Before it, there were only a few cloned sheep that were aborted, and countless time and energy were wasted.

If Yang Rui does not want to spend the next ten years on the same project, it is imperative to adopt a more economical method.

Thoroughly researching every key point is neither a correct choice, nor is it possible to achieve it, and it is even more unnecessary.

As long as Yang Rui's laboratory can complete the project of cloning sheep, there will naturally be someone to check and fill in the gaps that have not been clearly studied.

So many scholars from local universities, young people who have just started out, and poor labs in the third world have done this kind of work all their lives, and they never get tired of it, so they have to give others a chance.

Su Fan and Wang Liangcai also understood.

Yang Rui obviously disapproved of their step-by-step model, so Wang Liangcai couldn't help feeling a little anxious, and hurriedly said: "Professor Yang, Director Yang, cloning sheep is a series of projects, if the success rate in the early stage can't be guaranteed, you don't even have the qualifications to enter the later stage." .”

"Didn't Gu Qiang do the nuclear injection?"

"We can't learn Gu Qiang's method. He himself knows what it is but doesn't know why." Wang Liangcai sighed.

Yang Rui said calmly: "Then it's better to know what's going on and not know why."

"what?"

"We are in the same laboratory, we are in the same project team, and we are divided into different research groups just to try different paths. Now that Gu Qiang has gone through one path, let's use it, why waste experience on opening up a parallel path Up? My suggestion, until the part of the verification, you only carry out the work of checking and filling in the gaps, start from the verification, and continue to work hard."

Wang Liangcai was in a daze when he heard it, but he felt enlightened.

"You're right, there's no need to worry about why you use 5% concentration, as long as 5% concentration is easy to use." Wang Liangcai couldn't help but knocked himself on the head.

Has anyone studied an experiment thoroughly?

Of course there are, but those who have achieved this step are usually researched from the back to the front, that is, the later generations study the achievements of the predecessors.

Scholars who are really doing pioneering research and standing at the forefront of science are like foreigners exploring in the virgin rainforest. Who has the energy and time to explore the varieties and characteristics of each tree? Who has the ability to figure out the life under every inch of soil, or observe the movement in every tree crown?

Doing scientific research is like driving in a foggy day. You can't stop because you're waiting to be hit by the car behind you. Don't expect far visibility, you can see the road under your feet, and it's good not to fall down all at once.

Although Gu Qiang's experiment report lacks detailed analysis, there is one thing that is okay. He at least proved that the road is open.

"Success rate is still necessary, but there is no need to chase the limit, let's solve the problem of whether there is any." Yang Rui tantamount to overthrowing the original thinking of Wang Liangcai and Su Fan, but the two are not unhappy.

One should listen to valuable suggestions, which is the minimum attitude for scientific research.

It's useless to make strong orders with cells, and it's asking for trouble if you can't make ends meet with funds.

Wang Liangcai pondered for himself, and after a while, he said: "Since this is the case, let's repeat Gu Qiang's experiment first, and then extract the parts with obviously low success rate, and conduct in-depth research."

"In this case, Gu Qiang will still lead for a while." Su Fan was slightly unwilling.

Wang Liangcai took a look at Su Fan and said, "It's not fast first. Cloning sheep is a big project, just like long-distance running. Let's implement the follow-up strategy."

Compared with Su Fan, Wang Liangcai's personality is obviously more stable, and his method is also the best to take advantage of the two of them.

Moreover, compared to the silly method of building a foundation, the revised plan also takes into account both success rate and speed.

Most importantly, Gu Qiang's advantage also indirectly became the advantage of the two of them.

Yang Rui also nodded secretly. In this way, the efficiency of the laboratory can also be greatly increased.

No matter how lucky Gu Qiang is, he can't really get straight to the answer. From time to time, Yang Rui is always needed to correct his direction. The most important thing is that Gu Qiang's ability to expand scientific research is weak due to the lack of theoretical reserves.

Gu Qiang is not good at things like backtracking experiments, searching for missing parts, increasing the success rate, and improving the stability of experiments.

But in such a long project line of cloning sheep, these abilities are indispensable.

Wang Liangcai is just the opposite. He is not a scholar who is good at creating surprises, but a steady long-distance runner. Putting down a little bit of insignificant face, what Wang Liangcai got was the opportunity to strengthen and run fast.

"Professor Yang, we still want to apply for more time slots, especially the time slots at night." Wang Liangcai paused and said, "Can you give us all the time slots after midnight?"

"If you apply for the evening machine time, you must come every night." Yang Rui asked, "Can your body take it?"

"I can take a two-hour nap at noon, and four hours after dinner. In addition, I can sleep for about two hours the next morning, no problem." Wang Liangcai said it easily, but the three people present knew it. What a cruel perseverance to spread out the whole block of sleep time.

"Don't you need some...private time?" Yang Rui has seen those who work hard, and often asks the experimenters to work hard, but it is still rare to ask for super long working hours.

Wang Liangcai grinned and said, "My family is not here, what do I want to do in my private time?"

Yang Rui was speechless, shrugged, and said: "That's good, since your family is not in the capital, you can eat small stoves in the cafeteria. I will ask a nutritionist to prepare meals for you to ensure that they are delicious and nutritious. By the way, I also asked for a masseur a while ago, a national-level masseur, and when he arrives, I will allocate an hour to you every day."

Su Fan was about to drool enviously, but Wang Liangcai himself was at a loss.