Rebirth of the God-level Xueba

"So, Director Yang decided to reproduce this sheep by cloning from the very beginning?"

"Looks like it is."

"Then we will also conduct experiments on asexual reproduction in the future?" Su Fan's tone became more and more serious.

Wang Liangcai nodded solemnly with the same feeling, and said, "It seems so."

"Are you kidding me? Shouldn't it be... at least the sexual reproduction clone should be successful first, and then asexual reproduction?" Su Fan's thinking was almost confused.

Prior to this, when everyone talked about cloning, they were talking about sexual reproduction cloning, which is to put the nucleus of the germ cell into the enucleated egg cell.

So, cloning is not a new word.

However, even so, cloning is still a big project in genetic biology, such as the famous baby fish, which is a typical example of this kind.

In the development process of cloning, it basically follows the process from amphibians to aquatic products, then from aquatic products to mammals, and then from mammals to economic animals.

Both Su Fan and Wang Liangcai felt that it was time to clone economic animals, and they had the confidence and high spirit of a Terminator.

However, the sudden news of "clonal reproduction" blinded both of them.

This is equivalent to crossing several levels. It seems that children who have just started playing outdoors will go directly to Mount Everest without taking a helicopter.

Most importantly, biologists cannot prove that asexual reproduction is really feasible without previous similar experiments.

The normal model is to use an easier creature, first to prove that asexual reproduction is feasible, and then to be a mammal, which is the simplest and easiest solution.

But, looking at it another way, what creature is the easier creature?

Microbes don’t need to be considered, what about bigger fish and amphibians? Their gender is inherently ambiguous, and the situation of female fish changing into male fish happens frequently in nature.

The so-called, if you want to talk about sexually stable creatures, at least they must be mammals.

So, would smaller mammals be easier to clone? For example, the most common mice and rats in the laboratory?

No way!

Wang Liangcai, who had been experimenting for a period of time, came to this conclusion with a turn of his mind.

It takes a certain amount of time to carry out enucleation and nuclear injection. The embryo development time of mice is only two weeks, and that of sheep is six weeks. The latter is much easier than the former.

From this point of view, all animals commonly used in laboratories are not suitable for cloning. Because one of the reasons why the laboratory chooses them is the fast growth and development.

This is a good choice for experiments on adults, but when the experimental objects are embryos, these animals commonly used in laboratories are just not suitable.

Wang Liangcai raised his head and took a peek at Yang Rui, and said in a low voice, "Director Yang chose sheep as his first choice, and it's an idea of ​​getting it right once."

Su Fan is also a shrewd person. After thinking about it, he knew why Wang Liangcai said so, and said helplessly: "Director Yang is not a person who likes to follow the steps to do projects."

Recalling Yang Rui's previous projects, from the potassium ion channel experiment to the G protein-coupled receptor project, none of them were really done step by step.

Su Fan and the others didn't know Yang Rui's real support, so they can only evaluate it in this way in retrospect.

The so-called success is always a case study of success, and Wang Liangcai has no way to criticize it. He can only sigh and think, young people just can't hold back their temper.

However, such an idea can only be turned around in Wang Liangcai's mind. As far as Yang Rui's current reputation is concerned, it has long been separated from his age. People don't like to follow the steps, that is to forge ahead...

"What should we do now?" Su Fan was not a particularly thoughtful person, so he asked Wang Liangcai first.

"I have never done asexual clones. If it is feasible, the success rate will be extremely low." Wang Liangcai sighed.

The success rate is an eternal pain in the heart of experimental biologists. Many experiments are done knowing that they can be done.

Then the success rate was appallingly low, and I finally gave up.

Now when you encounter an experiment that may not be possible, and the success rate is as low as finger pointing, the result is very disgusting-you don't know whether your failure is a misoperation, or you can't do it.

At this time, Wang Liangcai was extremely eager to have a result prompt board to tell himself which path was doomed to fail.

"We have to increase the success rate in the early stage. The previous plan has to be overthrown and restarted." Wang Liangcai quickly made a decision, and said: "The success rate of enucleation must be increased to at least 80%, and then re-injection. "

Su Fan was not surprised that Wang Liangcai made this plan.

The step-by-step academic school is like this, it is impossible to fight for luck like Gu Qiang.

Pile up success rates on small, simple, and cheap projects, and the last-ditch scenario is the most common.

No one is going to change a bicycle into a motorcycle. The thinking of the academic school is that it is good to be able to change to a good bicycle. Besides, academics don’t ride motorcycles, do they?

Su Fan didn't mean to dissuade him, so he held a learning attitude and said, "If you want to improve the success rate in the early stage, do you want to do a comparative experiment?"

"Yes, to do a comparative experiment, it's best to find a few more people." Wang Liangcai said, looking around the laboratory.

Doing comparative experiments is actually doing small experiments.

For example, whether to use sucrose or no sucrose in the operating fluid, whether to use 2% sucrose or 3% sucrose, are all small details that cannot be smaller. However, the success rate of the experiment is the accumulation of such small details.

Why is it the same for peeling eggs? Some people have a success rate of 70 to 80 percent, while others have a success rate of only 20 to 30 percent. Apart from the difference in technique, it depends on who pays more attention to the details. Who knows more details.

Doing comparative experiments is to measure small details.

If you switch to a local university, you can publish a paper by doing such a comparative experiment.

A large number of Chinese journals and SCI entry-level journals publish papers of similar level. In fact, Yang Rui's first paper, the UV spectrophotometry of coenzyme Q10, was such an article, which solved a single problem in a narrow direction in related fields, so it could only be published in very low-level journals and became an authoritative paper. Information that can be found by scholars with relevant needs.

However, in computer retrieval, or in other words, when academic search engines have not yet appeared, even such data retrieval requires a lot of manpower and material resources.

Su Fan didn't need Wang Liangcai to assign tasks, and said first: "I'll go to the data team to urge me, and put the direction I'm looking for in the early stage?"

"Yes." Wang Liangcai's gaze was still roving the crowd.

Because Yang Rui's genetic engineering laboratory is newly built, the system adopted by Yang Rui is not the same as that of the ion channel laboratory. It adopts the system of a large laboratory, which allows researchers to join and form research groups freely, so it is more competitive.

However, setting up a task force requires money and time, and of course a task is also required.

The USD 600,000+30,000 that Gu Qiang fought for from Yang Rui, as well as the machine time of the inverted phase contrast microscope, belong to the resources of the research group.

Although the research group is still restricted and guided by Yang Rui, compared with the old model of the ion channel laboratory, the large laboratory system of the genetic engineering laboratory is obviously more independent and more suitable for the increasingly large scale. laboratory.

Theoretically, Wang Liangcai, who is on secondment, can recruit staff as long as he obtains the consent of other researchers. Anyway, it would be fine to list his name in the paper according to his contribution. The main interest of the laboratory lies here.

"In the old genetic engineering laboratory of Haidian District, researcher Sun Zhengchu has the strongest level, and his ability to cooperate is relatively good." Su Fan introduced Wang Liangcai in a low voice, then turned his head and said: "As for the experimental assistant, I think Qin Yue is good. , He used to be a veterinarian at China Animal Husbandry, and he has been seconded to the genetic engineering laboratory of Haidian District for a long time, and with our current project level, it is only a matter of one sentence to transfer him."

"This Qin Yue... is just an experimental assistant, wouldn't it be too troublesome to be transferred here?" Wang Liangcai hesitated a little.

Su Fan said carelessly: "I have the right to use it, and it will become invalid after the expiration date. We are now in the national project team. Think about how the previous projects of the same level were done? It is rare to call hundreds of people across the country. Now they want Zhongmu alone, they can't wait to send it over immediately."

Wang Liangcai was dumbfounded. He had been doing experiments in college before, and he had never participated in a project of this scale.

In biology, there are really not many so-called big projects, and they are all related to medicine. Genetic engineering has always been a niche, and Wang Liangcai has never really tried to criticize Fang Qiu.

"There must not be many experimental assistants. They are scarcer than researchers." Su Fan paused, and said, "It's hard to find good experimental assistants. Qin Yue has veterinary experience and knows how to work in the laboratory. I have also participated in the cattle embryo transfer project, so I have rich experience and hard work, which is very appropriate."

Wang Liangcai did have students under his command, but it was inappropriate to bring the National People's Congress all the way here, and experimental assistants were necessary.

As Su Fan said, a good experimental assistant is still indispensable. Sometimes, a test tube that is not cleaned may lead to the failure of the experiment, but a good researcher may not always clean the test tube.

"Just as you said. Let's second Qin Yue over here." After Wang Liangcai made the decision, he was in a good mood, and finally imitated the characters on TV, clenched his fists and said, "Let's have a big fight."

"Big fight." Su Fan laughed loudly.

With the laughter of the two, the laboratory also became noisy.

Wang Liangcai simply stopped and said with a smile, "Just go over to see how the others are doing."

"Okay." Su Fan followed Wang Liangcai through the public area where many instruments were displayed, and saw Gu Qiang surrounded by a group of people.

Su Fan frowned instinctively, and asked Tian Bing next to him, "What the hell is he doing?"

Tian Bing sighed and said: "You would never have imagined that Gu Qiang has never failed in nucleation and denucleation since the start of work. He has just done nucleation of breast cells and is now ready to inject nuclei."

"I haven't failed at one step..." Su Fan actually wanted to say that there is nothing special about not failing at one step, because if you fail one step later, all previous efforts will be wasted. Therefore, this is their original intention to continuously improve the success rate.

However, when the first half of this sentence was uttered, Su Fan couldn't say the second half.

In any case, Gu Qiang's current progress is the first in the laboratory.

"If his injection is successful, this will be the first case of successful injection in our laboratory." Tian Bing came to help, purely watching the excitement.

Su Fan laughed awkwardly, and was about to say something, but was interrupted by Gu Qiang's roar.

"Injection!" Gu Qiang stood up abruptly, wild like a rabbit who had completed the journey of life.

Everyone was also very happy to watch, clapping their hands and applauding, very happy for Gu Qiang's iron blood injection in the last five seconds.

Only Su Fan stared blankly ahead, as if something happened that he didn't want to recall.