Rebirth of the God-level Xueba

Wang Liangcai sat on the bed, staring at the envelope he received from the financial office, unwilling to move his eyes away for a long time.

The envelope is very thick, thick enough to doubt life.

JMC's dissertation bonus will not come until the dissertation is officially published, so Wang Liangcai is a little disappointed.

However, the prize money of the Achievement Award is obviously not comparable to the prize money for publishing a paper.

It was also seeing this envelope that made Wang Liangcai realize that the laboratory attaches great importance to the results.

After all, completing a project often represents multiple heavyweight papers. As for papers, if you want more, just write more, they are all attached to the results.

"One is Langyuan fairy flower, and the other is beautiful jade flawless. If there is no romance, I will meet him again in this life..."

The opening song of "Dream of Red Mansions" came from next door, which woke Wang Liangcai up violently.

The freshly released "Dream of Red Mansions" can be called the most attractive TV series of this year. The so-called empty alley is not an exaggeration at all.

When the opening song of the TV series plays, you can hear the sound of calling home in the yard. Adults and children who are crazy about playing are easily called home. You can’t go home if you don’t go home. After watching "Dream of Red Mansions", who will play with you?

There is no TV in Wang Liangcai's small suite, which is the only regret. Wang Liangcai went to the cafeteria to watch the big TV two days ago, but today he is too lazy to run away.

He reached out and grabbed the envelope on the bed, poured out the brand-new banknotes inside, counted it again, and then took out most of them and put them back in the envelope, thinking: My wife has wanted to change the color TV for a long time, otherwise, this year Just use this money in exchange.

The rest, Wang Liangcai divided it into three parts, and planned to keep one part for improved food for the family, another part to buy good clothes for his wife and children, and finally, some good books for himself.

After the allocation was completed, Wang Liangcai still couldn't believe it when he looked at the four piles of money in front of him.

He never had any monthly income, and he was able to do four things.

Not to mention four pieces, even a quarter of them, it's all very remarkable.

For example, when buying clothes, save some money every month, and only buy clothes once when the season changes, and you can’t buy them for everyone in the family. You must be buying autumn clothes for your wife this year, and you won’t have the money to buy winter clothes.

It is even more difficult to replace electrical appliances. It is common to have to save money for a year or two to buy an electrical appliance. During this period, there are no major projects that cost money.

Therefore, when you walk into ordinary people's homes, you can count the number of electrical appliances with one hand.

Wang Liangcai's family has no background. He has worked for many years and became a professor, so he bought a black and white TV, as well as an electric fan, a tape recorder and a single-tube washing machine, which can be regarded as catching up with the national average. Did not enter the scope of the discussion.

Wang Liangcai couldn't help touching the money with his hand, as if he was afraid that it would run away.

Turning his head, Wang Liangcai couldn't help laughing bitterly, he, a self-proclaimed literati, didn't expect that he also loves money so much.

"A gentleman loves money, and he gets it in a proper way." Wang Liangcai clenched his fists and made a secret decision. He hugged a pile of money into the quilt and fell into a dark sleep.

Four o'clock in the morning.

After sleeping for 8 hours, Wang Liang got up from the bed, washed up, and went to the laboratory.

The dormitory was very close to the laboratory, and when he reached the door, Wang Liangcai explained briefly, and the guard let him in without any surprise.

Wang Liangcai rubbed his hands as he walked. When he arrived at the laboratory, he felt his fingers were soft. He picked up the stove that was suppressed yesterday, added coal, changed into a lab coat, opened the instrument cabinet, and turned on the micromanipulator separately. Microscopes, piezo pulsers, stereo microscopes...

Superovulation and egg retrieval can only be regarded as the preparation stage for cloning sheep. Next, the real challenge begins with "denucleation".

Compared with the first two operations, the latter has a stronger sense of "scientific attributes".

At the very least, more instruments are used.

Judging from the prices of various equipment in 1987, there are only a handful of research institutes in China that can undertake this project.

Wang Liangcai inspected the instrument,

Deploy the nucleation solution smoothly.

There are two types of enucleation fluids: those without sucrose and those with sucrose. Various strange hormones can also be mixed into it. Every time you mix something into it, you can write a small paper.

Even the change of concentration can be regarded as a small subject.

But in Yang Rui's genetic engineering laboratory, such a project is naturally not eye-catching.

Wang Liangcai only made a version without sucrose and a version with 3% sucrose according to the traditional model. Immediately afterwards, he took out the petri dish, added a droplet of enucleation agent, added a cell relaxant, and then covered it with paraffin oil. Finally, he placed sheep oocytes in the microdroplet.

Just the small steps in the initial stage made Wang Liangcai sweat all over, and the chill in the laboratory had long since disappeared.

This is Wang Liangcai at the academician level. If it is another player, let alone a rookie, even an old professor who is known for his manipulation, or a new professor who is just a fledgling, they may not be able to play with these few steps.

At the very least, you have to fail for a week or two before you can complete the set action 100%.

Considering that every failure will waste one or more oocytes, one can imagine the importance of experienced players.

Not to mention, the next steps are just more complex continuous movements and more testing techniques.

Tong Dizhou was able to rise from the Belgian cattle back then because of his good skills.

As for why his technique is good? There is a story dedicated to it: At that time, the Belgian people had already begun to commute to get off work on time, and the scientific researchers strictly followed the work requirements, and went home to play after dinner. Drive people away.

This training method was passed down when Tong Dizhou returned to China.

"Have you ever seen the capital at four o'clock in the morning?" For genetics scholars, this is really not a question.

Otherwise, how to earn a reputation as a hard worker on the hard path of biology.

Wang Liangcai doesn't think it's hard to do experiments. Compared with metaphysics-like physics and suicide-like chemistry, biology is somewhat normal. At the very least, he can improve his own value through training.

The egg cells of sheep are too expensive, and they can be used as the egg cells of amphibious frogs like Comrade Di Zhou, a schoolboy.

However, long hours of training and concentration are definitely unavoidable.

Not only that, it is difficult for any experimenter to perfectly remember every experimental step, let alone keep abreast of the latest research trends in the scientific research community.

In this case, it is necessary to read a large amount of literature continuously.

Even with the help of the data group, the time saved is still limited.

Wang Liangcai also read the operation manual first, and then adjusted the lens of the inverted phase contrast microscope to align the oocyte, and gently waved the enucleation needle to find the correct position.

After rubbing his hands sorely, Wang Liangcai finally, with the help of a fixed needle, pressed the enucleated needle against the cells and sucked the zona pellucida, so that the enucleated needle maintained a negative pressure. Then he turned on the piezoelectric pulse device and tried to use a strong pulse to penetrate the transparent bring.

The nucleus is very small.

The cell is small enough, but the nucleus is smaller than the cell.

It is obviously not an easy task to remove the nucleus from the cell.

The blind suction method currently used by Wang Liangcai has a high success rate, but it is easy to suck too much of the eggs.

The latter is an important nutrient component for the development of egg cells into embryos. If too much is absorbed, the embryos will fail to develop fully.

Wang Liangcai also knew such disadvantages, so he thought while doing experiments.

Experiments are like this, there is no perfect experimental method, and it is impossible to stop continuing because the method is not perfect.

He will think about new methods, but if there is no moment of inspiration, it is not unacceptable to use stupid methods to produce results.

Unless the stupid method consumes too many resources, most experiments are completed by countless stupid methods connected with a few flashes of inspiration.

The sky was getting brighter and more people came to the laboratory, but Wang Liangcai continued to work without forgetting himself.

From four o'clock in the morning to eight o'clock in the morning, although he worked for four hours, as far as the experiment was concerned, he only carried out it four times.

And it failed four times.

For an experimental biologist accustomed to self-torture a thousand times, four times was just an appetizer.

At nine o'clock in the morning, Gu Qiang came to the laboratory unobtrusively.

He didn't arrive late, but he chose to go to the cafeteria to have breakfast first, and then came back after he was full, but it was the first time he saw Wang Liangcai who was working hard.

"It's too early." Gu Qiang couldn't help curling his lips, but his steps were three points faster.

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