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half a month later.

Chen Chen stopped the instrument in his hand and stretched out in front of the 100-level ultra-clean workbench, a look of excitement flashed in his eyes.

“The induction of fertilized egg clone No. 30 is completed and it can be implanted within the body of the mother.”

As Chen Chen stated, he began to fill in the experimental records and put it on his head. The experimental recorder faithfully records every frame of the picture, and these records will become the most precious data in the future.

At this time, the entire experiment is still in the early stage, that is, the cloning stage. Chen Chen needs to use a micromanipulator to remove a total of 36 egg cells from the nucleus and implant another nucleus to form a cloned fertilized egg.

This is also commonly known as “somatic cell nuclear transfer reprogramming.”

The road to cloning technology is not smooth. From 1938 when German embryologists first proposed the concept of cloning, to the birth of cloned sheep Dolly in 1996, and then to 2012 that humans discovered that caffeine can increase primordial length. The success rate of egg-like cell reprogramming, human cloning technology has been developed for nearly a century.

Until today, cloning technology has matured and commercialized. Many people are willing to spend a lot of money to clone dead pets and the emergence of profitable pet cloning companies are enough to show that cloning technology has matured day by day.

Because of this, Chen Chen was able to process dozens of SD rat cloned fertilized eggs in half a month.

However, mature technology does not mean simplicity.

What is cloning?

There used to be such a fable in the West:

It is said that there is a country where there is a toxic spring water. Anyone who takes a sip will go crazy and become a lunatic.

All the subjects of this country drank this spring water and fell into a state of madness, but the king did not drink it, so he did not go crazy with it.

As a result, the lunatics all over the country felt that the kings spirit had a problem, so they tied up the king and tried every means to treat his madness.

In the end, the king couldn’t stand the torture, so he could only take the initiative to drink the spring water and be crazy with the people…

The clone is the true reflection of this fable.

In a cell, there are dozens of materials such as cell membrane, cytoplasm, organelle, and cell nucleus. The most important of these is the cell nucleus, which is an organ that contains all the genetic information in the cell.

Generally speaking, in order for a cell to perform activities, it must first declare to the nucleus. After the nucleus is approved, it will call related genes, and then let a molecule called messenger RNA carry out the genetic information so that The relevant organelle executes its own commands.

It can be said that in the cell, the nucleus is the king, and the other structures and components in the cell are the subjects.

But if one day, scientists replace the king of cell nuclei, especially egg cells. Humans have removed the nucleus of the egg cell and stuffed the nucleus of the somatic cell of the object to be cloned into the egg cell instead.

Then, scientists used electric current and chemical agents to change the characteristics of this egg cell, turning it into a fertilized egg, just like letting the whole nation drink the poisonous spring water, so a scene in the fable happened.

Subjects asked the nucleus to give orders for the development of the fertilized egg, but the newly appointed nucleus refused. After all, he is not the nucleus of the egg cell. If you cant let me develop the fertilized egg, I will develop , My somatic cell nucleus is mutated, transformed into cancer cells, swallowed by white blood cells, and never develops!

However, with the continuous pressure of the subjects, this somatic cell nucleus finally felt “unbreakable” and had to change its position to transform itself into the nucleus of an egg cell.

At this point, the egg cell has successfully developed into an embryo, and the embryo will be implanted in a mother’s uterus, and a cloned life will eventually be born.

And this life, within the body, is flowing with exactly the same gene as the owner of the nucleus.

Clone successfully!

Of course, the actual operation is still very difficult. Not to mention the damage of the chemical agents, just replacing the nucleus on the egg cell is equivalent to a major operation.

After all, the egg cell needs to remove the cell nucleus first, and then implant the allogeneic cell nucleus. The egg cell is already dead if it is not killed on the spot.

I also took Chen Chen of NZT48 in order to maintain such an efficient success rate.

At this moment, the door behind Chen Chen was knocked and Wang Wei’s voice came in.

“Chenzi, the second batch of clones were born successfully, and no hidden diseases have been found for the time being.”

Unlike Chen Chens full spirit, Wang Weis voice is unconcealable tired.

“Okay, I will come right now.”

Chen Chen stood up and began to clean up the workbench in front of him. After freezing a few precious cloned fertilized eggs, he moved towards Go to another room.

As soon as he entered the door, Chen Chen heard a zhi zhi call. It was the call of a few newly born rats. Wang Wei and Xia Yin were surrounded by the cage and watched silently.

The whole body of the newborn rat is red, with only two black spots as large as sesame seeds in the eyes, which scream like a red worm.

“Get ready to start, extract neural stem cells to make cell suspension.”

“Okay.”

Wang Wei nodded, after a month of work, Wang Wei has been completely convinced by Chen Chen’s skill and perseverance. If it weren’t for Chen Chen, no student in the entire School of Biological Sciences could do this step.

Xia Yin on the side opened her mouth and looked at a bunch of newly born rats that didn’t even open their eyes, showing hesitation, “Are we really going to do it now?”

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“Since you have chosen this line, don’t be merciful.” Chen Chen indifferently said, “It is enough to respect this group of animals dedicated to science.”

“… ” Xia Yin was silent.

Immediately, the three of them began to compare the cloned mouse with the original owner. After confirming that there was no error, they were put to death.

The poor newborn rat has completely lost his life before he even opened his eyes to see this World.

“Is the ice PBS dish ready?” Chen Chen asked.

“Here.” Xia Yin brought a few petri dishes cautiously and looked towards Wang Wei, “You can start.”

“Okay.”

Wang Wei took the petri dish and began to execute the rat on the operating table, and took out the brain tissue from the rat’s carcass.

After that, he placed the rats brain in an ice PBS dish, and skillfully separated the hippocampus, put it in the buffer, and then turned around again to process the second rat as if it were an assembly line .

Chen Chen walked to the position before Wang Wei, cut the hippocampus into fine pieces, and added 0.25% pancreatin at the same time, and then placed it in a 37 degree incubator. Are you ready?”

“Ready.” Xia Yin took the test tube from the shelf.

Chen Chen nodded, turned around and started the operation on the second petri dish that Wang Wei had processed.

Xia Yin waited for two minutes, then took out the petri dish from the incubator, put it in the centrifuge for separation, and then added the planting solution and pipetted to form a cell suspension.

“di di di!”

Just then, the electronic alarm clock in the laboratory rang, and it was nine o’clock in the evening.

“Okay, let’s end the experiment for today.”

Although there is still unfinished work, Chen Chen has no intention of staying at all, so he just put down his hand. Tools, packed things up, turned and walked out of the laboratory.

“But we still have half an hour to finish today’s work?” Wang Wei puzzled.

“Work and rest are the most important.” Chen Chen shook the head, seriously said, “If you want to do this business for a long time, you can’t do without a good body.”

Chen Chen left, Wang Wei and Xia Yin looked at each other in blank dismay,

“Chen Chen is really strange.”

Xia Yin complained, “Working against time , Full of energy, one person can do the workload of several people, but once its the off-work time set by himself, he doesnt want to do one more second…”

“Maybe this is the quirk of scientists. “

Wang Wei shook the head, “Lets clean up too and do the rest tomorrow.”

“Okay.”

Xia Yin Nodded, the two left the laboratory one after another.